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Pregnancy education

AFP testing in the second trimester

Learn why second-trimester AFP screening may be offered, when to schedule it, and why an abnormal result does not confirm a birth defect.

Key points

  • Ask when your result will be reviewed and whether you need an additional appointment.

What is AFP screening

Alpha-fetoprotein, or AFP, is a protein made by your developing baby. A maternal blood test measures it to help screen for open neural tube defects, including open spina bifida. It can also suggest some abdominal wall defects. It does not detect every birth defect.

At Davie ObGyn, AFP testing is done between 15 and 20 weeks of pregnancy. Schedule within the specific dates your OB provides. No fasting is usually needed. MedlinePlus AFP information

Do I need AFP if I already had NIPT

NIPT screens for selected chromosome conditions. AFP addresses a different question, so it may still be offered after a low-risk NIPT result. AFP alone should not be treated as a second stand-alone Down syndrome screen. It may also be one part of a multi-marker blood test, which has a different purpose. Your OB will coordinate testing to avoid unnecessary duplicate chromosome screening. ACOG screening guidance

What does a high result mean

An elevated result is a reason to look more closely, not a diagnosis. Pregnancy dating, twins, and other factors can affect the number. Follow-up may include checking dates, a detailed ultrasound, counseling, and sometimes amniocentesis. The blood draw itself mainly carries minor risks such as soreness or bruising. MedlinePlus AFP information

Next step

Ask when your result will be reviewed and whether you need an additional appointment. Keep your anatomy ultrasound even when blood screening is reassuring.

Urgent symptoms? See when to call or go to the hospital. Emergency? Call 911.

Questions about your pregnancy? Contact Davie ObGyn in Davie, Florida, at 954-880-1010. New and established patients are welcome. This information supports your prenatal care and does not replace an individual medical assessment. For urgent symptoms, call your care team promptly; for an emergency, call 911. Do not use a routine online message for urgent symptoms.

Medically reviewed by Rahil Malik, MD, FACOG, board-certified obstetrician-gynecologist. Last reviewed: September 26, 2026. This page is general information and does not replace a visit with your doctor.

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