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Pregnancy education

Glucose screening and tolerance tests

Understand the one-hour glucose screen and fasting glucose tolerance tests, how to prepare, and what happens if a result is elevated.

Key points

  • Confirm the exact test and preparation with Davie ObGyn or your laboratory before the appointment.

Why we check blood sugar

Pregnancy can make insulin less effective, allowing blood sugar to rise. Gestational diabetes often causes no symptoms, so testing is usually offered at 24 to 28 weeks. Some patients need earlier testing based on their history. NIDDK testing guidance

Know which test you are having

TestWhat to expectPreparation
One-hour glucose challenge screenA 50-gram glucose drink, then a blood draw one hour laterFasting is generally not required; follow your lab's instructions
Three-hour glucose tolerance testA fasting sample, a 100-gram drink, and samples over three hoursFast as directed, usually at least eight hours; confirm water and medication instructions
Two-hour glucose tolerance testA fasting sample, a 75-gram drink, and additional samples over two hoursUsed by some practices; follow the specific fasting instructions

An elevated one-hour screen usually means a diagnostic test is needed. It does not automatically confirm gestational diabetes. The two-hour and three-hour tests use different diagnostic rules. NIDDK overview

Make the visit easier

Eat your usual diet in the days before testing; do not cut carbohydrates to try to “pass.” Ask how long to allow, what you may drink, and which medicines to take. Bring a snack for after a fasting test. Tell the team beforehand if you have had bariatric surgery or have trouble tolerating sweet drinks.

The drink may cause nausea, lightheadedness, or an upset stomach. Blood draws may bruise. If you vomit or feel unwell, tell staff; you may need another plan or a repeat test. MedlinePlus glucose testing information

Next step

Confirm the exact test and preparation with Davie ObGyn or your laboratory before the appointment.

Urgent symptoms? See when to call or go to the hospital. Emergency? Call 911.

Questions about your pregnancy? Contact Davie ObGyn in Davie, Florida, at 954-880-1010. New and established patients are welcome. This information supports your prenatal care and does not replace an individual medical assessment. For urgent symptoms, call your care team promptly; for an emergency, call 911. Do not use a routine online message for urgent symptoms.

Medically reviewed by Rahil Malik, MD, FACOG, board-certified obstetrician-gynecologist. Last reviewed: September 26, 2026. This page is general information and does not replace a visit with your doctor.

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